报告人：Guohong Peng
报告题目：Structures and Founctions of Membrane Protein: Structural MembraneProteomics on a Hyperthermophilic BacteriumAquifex aeolicus
时间：2012年4月12 日（星期四）10:00-11:30 am.
地点：生命科公司二楼大会议室
主持人：曹毅
报告人简介：
Ph. D. (Biology), Institute of Oceanology, Chinese Academy of Science, Qingdao, China, 1994;
Assistant researcher and Assistant Professor, Institute of Oceanology, Chinese Academy of Science, Qingdao, China, 1994-1999;
Groupleader,Department of Molecular Membrane Biology,Max-Planck-Institute of Biophysics, Frankfurt,Germeny, since 2004.
CURRENT RESEARCH：StructuralMembraneProteomics
For the structural proteomics projectofthe hyperthermophilic eubacterium,Aquifex aeolicushas beenselected because of the expectation of stable membrane protein complexes and because of the small size of its genome (less than 1/3 of that ofEscherichia coli) which substantially limits the number of possible membrane protein complexes in this bacterium.The aim of the project is to isolate,tocharacterize and to crystallize as many membrane proteins and membrane protein complexes as possible. Using conventional chromatographic methods we have purified and characterized the NADH quinone oxidoreductase (respiratory complex I), a possible supercomplex consisting of the cytochromebc1complex and of the cytochromecoxidase, the F1Fo-ATP synthase, the cytochromebdoxidaseand sulfid-quinone oxidoreductase, complexes of “hypothetical proteins”. For identification mass spectroscopy is used together with optical spectroscopy and enzymatic assays. A considerable percentage of the isolated protein complexes has yielded crystals.Several crystalsdiffracting X-rays to more than2 Å resolution have been obtained. Two of them had been solved the structures.
