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德国马克斯普朗克生物物理研究所Guohong Peng博士(主题:Structures and Founctions of Membrane Protein)4月12日学术报告

发布时间 :2012年04月06日 浏览量 :165

报告人:Guohong Peng

报告题目:Structures and Founctions of Membrane Protein: Structural MembraneProteomics on a Hyperthermophilic BacteriumAquifex aeolicus

时间:2012412 日(星期四)10:00-11:30 am.

地点:生命科公司二楼大会议室

主持人:曹毅

报告人简介:

Ph. D. (Biology), Institute of Oceanology, Chinese Academy of Science, Qingdao, China, 1994;

Assistant researcher and Assistant Professor, Institute of Oceanology, Chinese Academy of Science, Qingdao, China, 1994-1999;

Groupleader,Department of Molecular Membrane Biology,Max-Planck-Institute of Biophysics, Frankfurt,Germeny, since 2004.

CURRENT RESEARCHStructuralMembraneProteomics

For the structural proteomics projectofthe hyperthermophilic eubacterium,Aquifex aeolicushas beenselected because of the expectation of stable membrane protein complexes and because of the small size of its genome (less than 1/3 of that ofEscherichia coli) which substantially limits the number of possible membrane protein complexes in this bacterium.The aim of the project is to isolate,tocharacterize and to crystallize as many membrane proteins and membrane protein complexes as possible. Using conventional chromatographic methods we have purified and characterized the NADH quinone oxidoreductase (respiratory complex I), a possible supercomplex consisting of the cytochromebc1complex and of the cytochromecoxidase, the F1Fo-ATP synthase, the cytochromebdoxidaseand sulfid-quinone oxidoreductase, complexes of “hypothetical proteins”. For identification mass spectroscopy is used together with optical spectroscopy and enzymatic assays. A considerable percentage of the isolated protein complexes has yielded crystals.Several crystalsdiffracting X-rays to more than2 Å resolution have been obtained. Two of them had been solved the structures.